TwoStep Therapeutics

San Carlos, CA · Founded 2023 · Privately Held (backing)

Operator of a biotechnology company intended to develop novel tumor-targeting therapeutics for solid tumors. TwoStep Therapeutics is based in San Carlos, CA and was founded in 2023. Its last reported valuation was $14.5 million in Mar 2024. It has raised $51.5 million across 2 recorded rounds; the latest was a $21.1 million early-stage round in Jan 2026.

Last reported valuation
$14.5M
Oct 2026
Priced at
—
Total raised
$51.5M
Last round
Early Stage VC
Jan 2026

Who backs TwoStep Therapeutics?

7 investors
InvestorType
Insight Partners (New York)PE / buyout
NFXVenture capital
Alexandria Venture InvestmentsOther
2048 VenturesVenture capital
Medical Excellence CapitalVenture capital
Reference CapitalOther
GC&H Investments—
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TwoStep Therapeutics investors

7 investors are on record for TwoStep Therapeutics, including Insight Partners (New York), NFX, Alexandria Venture Investments and 2048 Ventures. The early-stage round in Jan 2026 drew 8 investors.

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