TwoStep Therapeutics

San Carlos, CA · Founded 2023 · Privately Held (backing)

Operator of a biotechnology company intended to develop novel tumor-targeting therapeutics for solid tumors. TwoStep Therapeutics is based in San Carlos, CA and was founded in 2023. Its last reported valuation was $14.5 million in Mar 2024. It has raised $51.5 million across 2 recorded rounds; the latest was a $21.1 million early-stage round in Jan 2026.

Last reported valuation
$14.5M
Oct 2026
Priced at
—
Total raised
$51.5M
Last round
Early Stage VC
Jan 2026
How much has TwoStep Therapeutics raised?
$51.5Macross 2 recorded rounds · Mar 2024 – Jan 2026

Most recently, TwoStep Therapeutics raised $21.1M in its Early Stage VC (Jan 2026).

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TwoStep Therapeutics round by round

2 rounds
Jan 2026Early Stage VC$21.1MSEC filing▮
8 investors participated
Mar 2024Early Stage VC$6.5MSEC filing▮
6 investors participated

How much has TwoStep Therapeutics raised?

TwoStep Therapeutics has raised $51.5 million in total; 2 of its rounds are on record. The largest on record was a $6.5 million early-stage round in Mar 2024; the most recent was a $21.1 million early-stage round in Jan 2026.

TwoStep Therapeutics has filed 1 Form D with the SEC, most recently in Mar 2024 ($6.5 million sold to 6 investors).

TwoStep Therapeutics investors

7 investors are on record for TwoStep Therapeutics, including Insight Partners (New York), NFX, Alexandria Venture Investments and 2048 Ventures. The early-stage round in Jan 2026 drew 8 investors.

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7 investors have backed TwoStep Therapeutics.

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Frequently asked about TwoStep Therapeutics

How much has TwoStep Therapeutics raised?
TwoStep Therapeutics has raised $51.5M in total across 2 recorded funding rounds.
When was TwoStep Therapeutics's last funding round?
TwoStep Therapeutics's most recent recorded round was Early Stage VC in Jan 2026, raising $21.1M.