Treeline Biosciences

Watertown, MA · Founded 2021 · Privately Held (backing)

Developer of transformative precision medicines intended to treat cancer and other serious conditions. Treeline Biosciences is based in Watertown, MA and was founded in 2021. It is valued at about $1.3 billion as of Oct 2026, set by institutional fund marks. It has raised $1.1 billion across 2 recorded rounds; the latest was a $200 million later-stage round in Aug 2025.

PMV mark · valuation
$1.3B
Oct 2026
Priced at
—
Total raised
$1.1B
Last round
Later Stage VC
Aug 2025
How much has Treeline Biosciences raised?
$1.1Bacross 2 recorded rounds · Oct 2024 – Aug 2025

Most recently, Treeline Biosciences raised $200.1M in its Later Stage VC (Aug 2025).

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Treeline Biosciences round by round

2 rounds
Aug 2025Later Stage VC$200.1MSEC filing▮
22 investors participated
Oct 2024Later Stage VC$421.8MSEC filing▮
43 investors participated

How much has Treeline Biosciences raised?

Treeline Biosciences has raised $1.1 billion in total; 2 of its rounds are on record. The largest on record was a $422 million later-stage round in Oct 2024; the most recent was a $200 million later-stage round in Aug 2025.

Treeline Biosciences has filed 2 Form Ds with the SEC since 2024, most recently in Sep 2025 ($200 million sold to 22 investors).

Treeline Biosciences investors

14 investors are on record for Treeline Biosciences, including GV, OrbiMed, ARCH Venture Partners and T. Rowe Price Group. By type, the record holds 4 venture capital firms, 1 hedge fund and 1 growth equity firm. The later-stage round in Aug 2025 drew 22 investors.

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Frequently asked about Treeline Biosciences

How much has Treeline Biosciences raised?
Treeline Biosciences has raised $1.1B in total across 2 recorded funding rounds.
When was Treeline Biosciences's last funding round?
Treeline Biosciences's most recent recorded round was Later Stage VC in Aug 2025, raising $200.1M.