Treeline Biosciences

Watertown, MA · Founded 2021 · Privately Held (backing)

Developer of transformative precision medicines intended to treat cancer and other serious conditions. Treeline Biosciences is based in Watertown, MA and was founded in 2021. It is valued at about $1.3 billion as of Oct 2026, set by institutional fund marks. It has raised $1.1 billion across 2 recorded rounds; the latest was a $200 million later-stage round in Aug 2025.

PMV mark · valuation
$1.3B
Oct 2026
Priced at
—
Total raised
$1.1B
Last round
Later Stage VC
Aug 2025
What is Treeline Biosciences worth?
$1.3BPrivateMarketView mark · Oct 2026

Reported valuation; nothing comparable since.

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The prices on Treeline Biosciences
  • ·18 fund marks: filer, period, implied $/share
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Valuation timeline

0 priced rounds · 32 daily marks since · Sep 2026 – Oct 2026
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Markrounds hold their value until the next priced eventMark, today
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Treeline Biosciences funding history

2 rounds
DateRoundRaisedPost-money
Aug 2025Later Stage VC$200.1M▮
Oct 2024Later Stage VC$421.8M▮
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Treeline Biosciences valuation

Treeline Biosciences is valued at approximately $1.3 billion as of Oct 2026. The figure is set by institutional fund marks.

Recent activity

In the last 90 days, Treeline Biosciences's record added 3 institutional fund marks (latest period Jul 2026).

How much has Treeline Biosciences raised?

Treeline Biosciences has raised $1.1 billion in total; 2 of its rounds are on record. The largest on record was a $422 million later-stage round in Oct 2024; the most recent was a $200 million later-stage round in Aug 2025.

Treeline Biosciences has filed 2 Form Ds with the SEC since 2024, most recently in Sep 2025 ($200 million sold to 22 investors).

Institutional holders and vehicles

2 SEC-registered funds reported 18 fair-value marks on Treeline Biosciences between Apr 2025 and Jul 2026, in their N-PORT filings. Filer-level marks and implied per-share values are available to Pro members.

How Treeline Biosciences's valuation is determined

Private company valuations aren’t quoted on an exchange — they’re established in moments: a priced funding round sets a post-money valuation, secondary trades print at a price per share, and SEC-registered funds holding the stock report fair-value marks in their N-PORT filings.

For Treeline Biosciences, the most current credible signal sets the number and the older ones confirm it: a live secondary-market price first, then three or more recent prints, then institutional fund marks, and otherwise the last priced round, which never decays. The valuation is the last round’s post-money moved by the price change since that round, so no share count is assumed. Today it is set by institutional fund marks. Question about this number? Full methodology →

Frequently asked about Treeline Biosciences

What is Treeline Biosciences worth?
Treeline Biosciences is valued at $1.3B as of Oct 2026. Reported valuation; nothing comparable since.
How much has Treeline Biosciences raised?
Treeline Biosciences has raised $1.1B in total funding across 2 recorded rounds.
When did Treeline Biosciences last raise?
Treeline Biosciences last raised in Aug 2025 (Later Stage VC), raising $200.1M.