Trefoil Therapeutics

San Diego, CA · Founded 2013 · Privately Held (backing)

Developer of corneal therapeutics designed to facilitate the treatment of ophthalmic diseases. Trefoil Therapeutics is based in San Diego, CA and was founded in 2013. It was valued at $59.5 million at its later-stage round in May 2025, the last priced event on record. It has raised $49.7 million across 4 recorded rounds; the latest was a $4.6 million seed round in Apr 2025.

PMV mark · valuation
$59.5M
Oct 2026
Priced at
its later-stage round
May 2025
Total raised
$49.7M
Last round
Seed Round
Apr 2025
What is Trefoil Therapeutics worth?
$59.5MPrivateMarketView mark · Oct 2026

Valued at $59.5M at its later-stage round, May 2025. Nothing has traded since.

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Valuation timeline

0 priced rounds · 32 daily marks since · Sep 2026 – Oct 2026
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Trefoil Therapeutics funding history

4 rounds
DateRoundRaisedPost-money
Apr 2025Seed Round$4.6M▮
Mar 2022Early Stage VC$12.0M▮
Jul 2019Early Stage VC$12.6M▮
Dec 2016Seed Round$4.2M▮
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Trefoil Therapeutics valuation

Trefoil Therapeutics was valued at $59.5 million at its later-stage round in May 2025. That is the last priced event on record: no secondary trade or institutional fund mark has moved the figure since.

How much has Trefoil Therapeutics raised?

Trefoil Therapeutics has raised $49.7 million across 4 recorded rounds since 2016. The largest was a $4.6 million seed round in Apr 2025.

Trefoil Therapeutics has filed 5 Form Ds with the SEC since 2016, most recently in May 2025 ($4.6 million sold to 16 investors).

How Trefoil Therapeutics's valuation is determined

Private company valuations aren’t quoted on an exchange — they’re established in moments: a priced funding round sets a post-money valuation, secondary trades print at a price per share, and SEC-registered funds holding the stock report fair-value marks in their N-PORT filings.

For Trefoil Therapeutics, the most current credible signal sets the number and the older ones confirm it: a live secondary-market price first, then three or more recent prints, then institutional fund marks, and otherwise the last priced round, which never decays. The valuation is the last round’s post-money moved by the price change since that round, so no share count is assumed. Today it is set by its last priced round. Question about this number? Full methodology →

Frequently asked about Trefoil Therapeutics

What is Trefoil Therapeutics worth?
Trefoil Therapeutics is valued at $59.5M as of Oct 2026. Valued at $59.5M at its later-stage round, May 2025. Nothing has traded since.
How much has Trefoil Therapeutics raised?
Trefoil Therapeutics has raised $49.7M in total funding across 4 recorded rounds.
When did Trefoil Therapeutics last raise?
Trefoil Therapeutics last raised in Apr 2025 (Seed Round), raising $4.6M.