Trefoil Therapeutics

San Diego, CA · Founded 2013 · Privately Held (backing)

Developer of corneal therapeutics designed to facilitate the treatment of ophthalmic diseases. Trefoil Therapeutics is based in San Diego, CA and was founded in 2013. It was valued at $59.5 million at its later-stage round in May 2025, the last priced event on record. It has raised $49.7 million across 4 recorded rounds; the latest was a $4.6 million seed round in Apr 2025.

PMV mark · valuation
$59.5M
Oct 2026
Priced at
its later-stage round
May 2025
Total raised
$49.7M
Last round
Seed Round
Apr 2025
How much has Trefoil Therapeutics raised?
$49.7Macross 4 recorded rounds · Dec 2016 – Apr 2025

Most recently, Trefoil Therapeutics raised $4.6M in its Seed Round (Apr 2025).

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Trefoil Therapeutics round by round

4 rounds
Apr 2025Seed Round$4.6MSEC filing▮
16 investors participated
Mar 2022Early Stage VC$12.0MSEC filing▮
14 investors participated
Jul 2019Early Stage VC$12.6MSEC filing▮
13 investors participated
Dec 2016Seed Round$4.2MSEC filing▮
14 investors participated

How much has Trefoil Therapeutics raised?

Trefoil Therapeutics has raised $49.7 million across 4 recorded rounds since 2016. The largest was a $4.6 million seed round in Apr 2025.

Trefoil Therapeutics has filed 5 Form Ds with the SEC since 2016, most recently in May 2025 ($4.6 million sold to 16 investors).

Trefoil Therapeutics investors

12 investors are on record for Trefoil Therapeutics, including National Institutes of Health, CONNECT(San Diego), Correlation Ventures and Hatteras Venture Partners. The seed round in Apr 2025 drew 16 investors.

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12 investors have backed Trefoil Therapeutics.

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Frequently asked about Trefoil Therapeutics

How much has Trefoil Therapeutics raised?
Trefoil Therapeutics has raised $49.7M in total across 4 recorded funding rounds.
When was Trefoil Therapeutics's last funding round?
Trefoil Therapeutics's most recent recorded round was Seed Round in Apr 2025, raising $4.6M.